Final blood cultures were negative. After incubation of the epiglottic swab specimen on 5% sheep blood agar plates at 37°C in 5% carbon dioxide, the predominant colonies found were large and beta ...

Culture is necessary in all cases of severe corneal ulcers and suspected fungal infection. For isolation of bacteria and fungi, BA and chocolate agar (CA) may be used.

Throat cultures, nasal cultures and cultures of impetiginous lesions on the scalp or face were inoculated on chocolate and sheep-blood agar plates. Needle aspiration of all nodes was performed.

A volume of 100 μL of each dilution was plated on Columbia agar with sheep blood (Thermo Fisher Scientific GmbH, Wesel, Germany) and incubated for 24 h at 37°C under aerobic conditions. Bacterial ...

B. pertussis was grown on Bordet-Gengou agar (BD, Sparks, MD, USA) supplemented with 10% casamino acid (Fisher Scientific, USA). B. holmesii and B. parapertussis were grown in 5% sheep blood agar (BD, ...