Three former athletes at the University of Pennsylvania women’s swimming program sued the school, the Ivy League Council of Presidents and athletics organizations Tuesday, claiming they violated federal law to allow transgender athlete Lia Thomas to compete against them.

The new policy, which is effective immediately, limits competition in women’s sports exclusively to athletes assigned female at birth.

The decision, effective immediately, came a day after President Trump signed an order barring transgender girls and women from playing in women’s sports at federally funded educational institutions.