Sirius red stain confirmed a significant (p; 0.1, Wilcox Ranksum) increase in collagen content in the fibrotic medulla and cortex relative to control. These results suggest that VisR noninvasively ...

To quantify the % of fibrotic regions, 2D RGB images (8 bits per channel) of Sirius Red and Trichrome (without the hematoxylin stain) were read into the Fiji version 76 based on ImageJ2 77. “Analyze ...

ECM collagen fibers were red-stained by Sirius red/fast green staining and blue-stained by Masson’s trichrome. ... Cell Signaling Technology, RT, 2h), and then incubated with ...

Five micrometers paraffin sections were stained using Alexa Fluor 488 conjugated wheat germ agglutinin (WGA; Invitrogen) to identify myocyte cell borders or Picro Sirius Red Stain Kit (Polysciences) ...

To measure collagen abundance, extracted ECM was stained with the Picro Sirius Red Stain Kit (Abcam) according to the manufacturer's instructions. The stain was extracted with 0.1 M NaOH, and optical ...

Chronic kidney disease is associated with progressive inflammation and fibrosis, which is conventionally assessed by invasive biopsy. A noninvasive alternative to biopsy may be interrogating elastic ...

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Collagen was typed and quantified using the picrosirius ... fragments were cut at 5 µm thickness and colored in Harris’s hematoxylin and eosin and Mallory’s trichrome stains. ... W. and Brentani, R.R.