To evaluate SYBR Green I real-time RT-PCR for the universal detection of flaviviruses, several commercial kits were tested. The KAPA SYBR FAST Universal 2X qPCR Master Mix kit exhibited the maximal ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed ...

Megan Burch and Jeremy Bechelli. Development of a SYBR Green-Based RT-qPCR Assay for the Detection and Quantification of Lone Star Virus. Zoonoses. 2024. Vol. 4 (1). DOI: 10.15212/ZOONOSES-2023-0037 ...

The resulting DNA was subjected to qPCR using PowerTrack SYBR Green qPCR Mix (Thermo Fisher Scientific, Inc.) according to the manufacturer’s specifications, with the exception of a final primer ...

So, what can cause green pee? While green pee, once again, isn't something to be alarmed over, you'll likely be comforted by knowing that you can tie it to certain behaviors if you do experience it.

The duplex qPCR was performed using the following optimized reaction mix of 12.5 µL Luminoct ® SYBr ® Green qPCR Readymix (Sigma-Aldrich, St. Louis, Missouri, USA), 0.05 µM of the primer pSE, 0.025 µM ...