Frontiers1y
A novel glycolysis-related gene signature for predicting the prognosis of multiple myeloma
Finally, the expression of the selected genes was further verified by quantitative real-time PCR (qRT-PCR). The prognostic glycolytic ... Consistent with previous results, the pathways of cell cycle, ...
Frontiers1y
Identification and validation of a novel cuproptosis-related gene signature in multiple myeloma
Finally, we collected MM cell lines and patient samples to validate marker gene expression by quantitative real-time PCR (qRT-PCR ... was calculated using the comparative threshold cycle (Ct) method ...
eLife2y
Prevalent and dynamic binding of the cell cycle checkpoint kinase Rad53 to gene promoters
Cell cycle regulated, genome-wide binding of Rad53 to chromatin was examined. Under replication stress, the kinase bound to sites of active DNA replication initiation and fork progression, but ...
eLife11y
Mechanism for priming DNA synthesis by yeast DNA Polymerase α
The DNA Polymerase α (Pol α)/primase complex initiates DNA synthesis in eukaryotic replication. In the complex, Pol α and primase cooperate in the production of RNA-DNA oligonucleotides that prime ...