Crystal violet showed about the same selective and bactericidal action as gentian violet. 3. Methylene blue as made up for staining showed little or no bactericidal action, the percentage of alcohol ...
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size and arrangement of bacteria. 1. Use the smear prepared in the previous procedure. Staining is done at the sink. 2. Add several drops of Methylene blue, enough to cover the smear, and wait 1 min.
Originally synthesized in 1876, methylene blue is a diaminophenothiazine that has a low redox potential of 11 mV, thereby allowing this compound to readily cycle between its oxidized and reduced ...
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