These reactions can be used for the selective labeling of azide-functionalized biomolecules. The primary advantage of the catalyzed azide-alkyne cycloaddition over the Staudinger ligation is its ...

Remarkably, this chemistry transforms lysine, a nucleophile, into an electrophilic warhead. We demonstrate its versatility in late-stage peptide diversification, precision protein engineering, and ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. High-throughput measurement of cellular traction forces at the nanoscale remains a ...

During the peer-review process the editor and reviewers write an eLife assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) ...

Cells were treated for 1 minute with 1 mM H 2 O 2 to initiate biotinylating reaction and washed three times with Quencher solution (10 mM sodium ascorbate, 5 mM Trolox, 10 mM sodium azide in DPBS).

s estimation of 25%–50% PEG conjugation efficiency onto PVX, another high-aspect-ratio plant virus, in which there was estimated to be one grafted PEG for every three lysine residues (49). Purified ...

The development of targeted medicine has greatly expanded treatment options and spurred new research avenues in cancer therapeutics, with monoclonal antibodies (mAbs) emerging as a prevalent treatment ...