After a washing step, cells were stained using a working solution containing the dyes Calcein-AM (2 μM) and ethidium homodimer-1 ... The CellTiter-Blue Cell Viability Assay (Promega, Madison, WI, USA) ...

The working solution contained 2 mL of assay buffer, 1 μL of Calcein AM (1000×), and 1 μL of PI (1000×) and 250 μL of this were placed in each well, followed by incubation at 37 °C for 30 min in the ...

Cholesterol levels were quantified using an enzymatic assay (c.f.a.s., Cobas ... After tubular network was formed by HAECs, cells were stained with 6 µmol/L Calcein AM solution (R&D Systems) for 15 ...

The PLA assay is a robust method for detecting proteins located ... Cells were then incubated with 1 μM calcein-AM (Thermo Fisher Scientific, MA, United States), washed with PBS, and imaged using a ...

On 21 December 1988, flight Pan Am 103 was en route from Heathrow to JFK when a bomb exploded in its hold over Lockerbie, killing 270 people, including 43 British citizens and 190 Americans.

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Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...