These peptide pools were dissolved in dimethyl sulfoxide (DMSO, Sigma) at a concentration of 0.4 mg/ peptide ... The sPD1-SIVgag gene was created by fusing the N-terminal region of mouse soluble PD1 ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

Reviewers have tested some of the products below and may have received some products from the manufacturers for free. All opinions are the reviewers’ own. These full-spectrum gummies are ...

The reaction was either performed with the indicated concentrations of peptides or DMSO (Sigma Aldrich) as the vehicle control. The reaction was incubated for 30 min at 23 °C and stopped by the ...

Department of Chemistry, University of Toronto, Lash Miller Chemical Laboratories, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada Acceleration Consortium, University of Toronto, 700 University ...

Spectral flow cytometry is an upcoming technique that allows for extensive multicolor panels, enabling simultaneous investigation of a large number of cellular parameters in a single experiment. To ...

All cell culture media, buffers, and other laboratory chemicals were obtained from Sigma-Aldrich (Gilligham ... Both compounds were dissolved in 100% DMSO and then diluted in cell media to reach their ...

The most common method for creating an amide bond is the use of carbodiimide condensing agents such as dicyclohexylcarbodiimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. The ...

To exclude off-target effects of siRNAs targeting GPx4 for the drop of HCV infectivity, the isoform specificity of these siRNAs targeting GPx4 was validated (figure 2G), and the effect of additional ...

Several species of the genus Euphorbia (Eu-phorbiaceae) have been tested for their efficiency as antiviral and antitumour agents, partly based on information concerning plants that have traditionally ...