Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

Methotrexate does not produce a response if it doesn’t eliminate enough malignant B-cells. Flow cytometry can count these cells. If the technology sees that the methotrexate didn’t adequately ...

However, such experiments were problematic for flow cytometry as there weren’t enough different fluorophores available to identify multiple cell phenotypes at the time. Researchers turned their ...