BioTechniques22d
Faster, cheaper, simpler: flow cytometry just got an upgrade
This small device could make flow cytometry more ... relies on laborious flow cytometry techniques, making this a huge potential saving for researcher’s time and funding. “Identifying and quantifying ...
Frontiers29d
BKV Clearance Time Correlates With Exhaustion State and T-Cell Receptor Repertoire Shape of BKV-Specific T-Cells in Renal Transplant Patients
Here we apply a combination of in-depth multi-parametric flow cytometry and NGS-based CDR3 beta chain receptor repertoire analysis of BKV-specific T-cells to a cohort of 7 kidney transplant patients ...
Frontiers29d
Ligand-based targeting of c-kit using engineered γδ T cells as a strategy for treating acute myeloid leukemia
Cells were then washed once with FACS buffer, then subjected to flow cytometry (Cytek Aurora) and analyzed with FlowJo software (v10). Jurkat or primary CAR T cells were co-cultured with VPD450 (BD, ...
Frontiers29d
Treg in inborn errors of immunity: gaps, knowns and future perspectives
Regulatory T ... flow cytometry-optimized assays using fluorescent reactive amines such as CFSE (Carboxyfluorescein succinimidyl ester) are more efficient and accurate. CFSE labelling of Teff allows ...
rarediseaseadvisor29d
Diffuse Large B-Cell Lymphoma (DLBCL)
Flow cytometry is used to analyze the cell surface markers CD45, CD3, CD5, CD19, CD10, CD20, and kappa/lambda. 2,9 Cytogenetics or FISH assesses for chromosomal translocations to identify t(14;18), ...