Mutations that disrupt the trpR gene lead to elevated production ... can once again bind to the operator/promoter, quickly turning off expression of the operon, returning expression to basal ...

The answers to such questions lie in the study of gene expression. Thus ... bind near the start of mRNA production for an entire operon, or cluster of coregulated genes. Furthermore, some ...

Genetic engineering in non-human primates has long been limited by the need for virus-based gene delivery methods. Recently, ...

Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA sequence loxP, which is its target. This vector allows the creation of ...

They inserted these two sequences upstream of a reporter gene and used a colorimetric assay to determine whether the proteins repressed the reporter’s expression based on an absence of color. Their ...