For profiling macrophages, the gain was set to: FSC: 53, SSC: 21, APC: 131, PE: 27. A total of 10k events were collected. At minimum, 3000 events in the APC+ gate were collected. Antibodies used for ...

The percentage of alveolar macrophages that had undergone efferocytosis was determined by flow cytometry. We conducted a multivariable ... The flow cytometric gating strategy (see online supplementary ...

NAMPT blockage influenced macrophage differentiation processes ... Mouse LPMNCs were isolated and analysed by flow cytometry (gating strategy outlined in online supplementary figure 2A,B). In ...

The retrieved cells were expanded in culture and characterized based on their phenotype, immunomodulatory effects on lymphocytes and macrophages ... draft and Writing–review and editing. All flow ...

Flow cytometry panel building can be daunting ... Once identified, choose key markers that best define these cells and establish a hierarchical gating strategy to refine their selection. You can also ...

Beckman Coulter Life Sciences, a global leader in laboratory automation and innovation, and an Operating Company of Danaher Corporation, revolutionizes spectral flow cytometry with the industry ...

This study provides proof-of-principle data for the use of trained immunity to modulate macrophage interactions with tumours. The study makes a valuable contribution to the field of trained immunity.

To support physicians in treating cancer patients, oncology laboratories can benefit from adopting end-to-end automation of flow cytometry workflows. Rapid advances in automation are improving ...

CD47-amyloid-β-CD74 signaling is a key driver of adaptive immunosuppression in sepsis, suppressing B cells and impairing ...