A novel molecular thermometer based on color-changing fluorescent dyes achieves unprecedented sensitivity and resolution when ...

For a single fluorescent molecule, the signal is zero if and only if the zero-intensity node of the ... able to separate two permanently emitting fluorophores at a distance of down to 8 nanometers.

Temperature is a critical variable that influences countless biological processes at the cellular level. However, precisely measuring temperatures ...

For a single fluorescent molecule, the signal is zero if, and only if, the zero-intensity node of the illumination beam ... For example, they were able to separate two permanently emitting ...

At the cellular level, temperature is a crucial factor that affects innumerable biological processes. It is still difficult to measure temperatures inside living cells precisely, though.

One such technique, termed fluorosequencing, integrates Edman degradation, single-molecule microscopy and stable fluorophore chemistry and multiplexing ... amino acid and thereby alters the ...

The study introduces new tools for measuring the intracellular calcium concentration close to transmitter release sites, which may be relevant for synaptic vesicle fusion and replenishment. This ...