The plates for enumerating LAB (De Man Rogosa Sharpe agar culture media) were placed in an anaerobic box (Anaerobic box; C-31, Mitsubishi Gas Chemical Co., Tokyo, Japan) and incubated at 37°C for 48 h ...
Bacteria were then resuspended in warm (50°C), sterile underlay agar [10 mM sodium phosphate buffer, 1% (v/v) Mueller-Hinton broth, 1.5 (w/v) agar] at 4 × 10 5 colony forming units per mL. Fifteen ...
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