BACKGROUND: Despite the high morbidity and mortality of heart failure with preserved ejection fraction (HFpEF), treatment options remain limited. The HFpEF syndrome is associated with a high ...

This study provides a potentially useful investigation into the positive role of BDNF/TrkB signaling in implanted dental pulp stem cells to enhance dentin regeneration in the context of dental caries.

and DAPI (C1006, Beyotime, Shanghai, China) was used for nuclear staining. The cell morphology was observed using an inverted fluorescence microscope. The experimental animals were randomly divided ...

G) Zoom-in of boxed region in (A), with DAPI-stained nuclei and notum FISH signal shown separately ... their splenocytes were then used to create clonal hybridoma cell lines. Clone supernatants were ...

Briefly, in DENV-infected BHK-21 cells, we fixed and stained the infected cellular monolayer by using ... Cells were counterstained with 1 μg/ml DAPI in PBS for 5 min and washed three times with PBS.

Slides were subsequently washed and mounted using Vectashield containing 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) (Vector Labs). For cell immunofluorescence staining, 5000 normal ...

Marek's disease can be pervasive in chicken houses. Chickens infected by this highly contagious viral disease often develop ...

The team from The Florey and University of Melbourne focused on somatic mutations, which are not inherited, but occur over time in cells during aging. Mutations can disrupt a cell's normal ...

After fixation with paraformaldehyde, cells were incubated with a 1:2,000 dilution of anti-PS monoclonal antibody clone PS05 (green) in PBS followed by counterstaining of nuclei with DAPI (blue).

e) A stepwise scheme obreast cancer progression (normal (MCF10A (M1)), premalignant (MCF10AT (M2)) and malignant (MCF10CA1h (M3)) breast cells) and its characterization with f–h) DAPI staining ...