Also 10-N-nonyl-acridine orange (NAO) is a cell permeant dye. This probe accumulates into mitochondria and binds to phospholipids and, particularly, to mitochondrial cardiolipin 7.

Figure 1. Comparison of viability counts using either brightfield (trypan blue) or fluorescence (acridine orange / propidium iodide) methods. Image Credit: DeNovix Inc.

Pharmacokinetic Evaluation of N-[2-(Dimethylamino)Ethyl]Acridine-4-Carboxamide in Patients by Positron Emission Tomography Authors : Azeem Saleem , Robert J. Harte , Julian C. Matthews , Safiye Osman ...