eLife18d
Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction
Department of Anatomy and Cell Biology, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States Thomas Jefferson High School for Science and Technology, ...
C&EN19d
Mapping Tumor–Stroma–ECM Interactions in Spatially Advanced 3D Models of Pancreatic Cancer
Centre for 3D models of Health and Disease, Division of Surgery and Interventional Science, University College London, London W1W 7TY, U.K. Centre for 3D models of Health and Disease, Division of ...
TechRadar28d
Polycam pulls in the real world and makes sense of it in 3D – and I am hooked
It’s a strange dichotomy, existing in a 3D world but living and working in 2D spaces. Combining the real 3D dimensional world with the digital 2D one isn’t easy. Mixed reality tries to do it ...
Frontiers3y
From Toxicity to Selectivity: Coculture of the Fluorescent Tumor and Non-Tumor Lung Cells and High-Throughput Screening of Anticancer Compounds
The calcein AM assay was based on the live and dead assay protocol (ThermoFisher). 2,500 cells per well for the A549’ cell line or 4,000 cells per well for the VA-13 cell line were plated out in 140 ...
Frontiers4y
Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance
Cells were incubated with 1 μM non-fluorescent calcein-AM for 15 min at 37°C to confirm cellular metabolism ... In addition, a leakage assay was performed with rhombus-shaped scaffolds to assess ...
eLife4y
Zinc shapes the folding landscape of p53 and establishes a pathway for reactivating structurally diverse cancer mutants
Missense mutations in the p53 DNA-binding domain (DBD) contribute to half of new cancer cases annually. Here we present a thermodynamic model that quantifies and links the major pathways by which ...