The back-to-back deaths of two beloved bald eagle siblings stunned the thousands of people who tuned into the livestream of their nest in Florida. Now, biologists have shared they both died from bird flu. The bald eaglets, known as E24 and E25, died within 36 hours of each other, McClatchy News previously reported.

The two eaglets from the Southwest Florida Eagle Cam that died within a day of each other tested positive for Avian Influenza.

The eagle cam team says E25 started to have seizures similar to those of E24 and fell out the nest around 3 a.m. on Tuesday.