We chose to use the Sirius Red stain to better visualize the assembled collagen fibers, since degraded collagen fragments is also reflected in the hydroxyproline assay. 12 The H&E staining was ...

Red color is fat (Oil Red O) and green is collagen (Picro Sirius Red). This image relates to a paper that appeared in the July 31, 2013, issue of Science Translational Medicine, published by AAAS.