In Sanger sequencing, the DNA to be sequenced serves ... and T reactions are individually loaded into four lanes of a single gel and separated using gel electrophoresis, a method that separates ...
Sanger sequencing (left) sequences 500-700 bases per reaction with 16 reactions per gel. Massively parallel DNA sequencing (center) sequences 100-5,000 bases per reaction with 10 thousand to 10 ...
When Sanger sequencing was first introduced, four separate reagents were used, one for each type of ddNTP. The four reaction products were then separated by gel electrophoresis, a process that ...
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