It is analogous to Southern blotting, which is used to analyze DNA. This protocol describes ... alkaline transfer. Stain with methylene blue (see below). Uncharged or positively charged nylon ...

What is methylene blue? Methylene blue is a synthetic dye and medication used for a variety of purposes, including treating blood conditions, staining cells, and detecting pathogens. Considered ...

1. Use the smear prepared in the previous procedure. Staining is done at the sink. 2. Add several drops of Methylene blue, enough to cover the smear, and wait 1 min. 3. Rinse the slide with water from ...