the fluorescence of the probe is quenched when bound to a “cage”, but upon irradiation of light at specific wavelength, the “cage” is cleaved, releasing the fluorophore and restoring its ...
By conjugating luciferase protein to quantum dots, bioluminescence resonance energy transfer (BRET) turns these useful fluorophores into a new class of bioluminescent probe. Quantum dots have ...
Such probes consist of a 5' fluorophore dye, a 3' Black Hole Quencher (BHQ), and an internal nova quencher integrated between base residues 9 and 10. Image credit: LGC Biosearch Technologies When ...
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