investigators will also stab several times through the agar using an inoculating loop. This stab allows for the detection of streptolysin O, a specific hemolysin produced by Streptococcus pyogenes.

This agar is used to identify organisms that are capable of producing ... The plate pictured on the left is lipase negative. 1. Collect your inoculating loop, as well as your Gram-positive Unknown A ...

It is therefore important that any petri dish or agar used is sterilised before use, and that aseptic techniques are used to inoculate the plates. An inoculating loop can be used to transfer bacteria.